Artificial neural networks and physical modeling for determination of baseline consumption of CHP plants

An effective modeling technique is proposed for determining baseline energy consumption in the industry. A CHP plant is considered in the study that was subjected to a retrofit, which consisted of the implementation of some energy-saving measures. This study aims to recreate the post-retrofit energy consumption and production of the system in case it would be operating in its past configuration (before retrofit) i.e., the current consumption and production in the event that no energy-saving measures had been implemented. Two different modeling methodologies are applied to the CHP plant: thermodynamic modeling and artificial neural networks (ANN). Satisfactory results are obtained with both modeling techniques. Acceptable accuracy levels of prediction are detected, confirming good capability of the models for predicting plant behavior and their suitability for baseline energy consumption determining purposes. High level of robustness is observed for ANN against uncertainty affecting measured values of variables used as input in the models. The study demonstrates ANN great potential for assessing baseline consumption in energyintensive industry. Application of ANN technique would also help to overcome the limited availability of on-shelf thermodynamic software for modeling all specific typologies of existing industrial processes

Optimization of a Steam Reforming Plant Modeled with Artificial Neural Networks

The objective of this research is to improve the hydrogen production and total profit of a real Steam Reforming plant. Given the impossibility of tuning the real factory to optimize its operation, we propose modelling the plant using Artificial Neural Networks (ANNs). Particularly, we combine a set of independent ANNs into a single model. Each ANN uses different sets of inputs depending on the physical processes simulated. The model is then optimized as a black-box system using metaheuristics (Genetic and Memetic Algorithms). We demonstrate that the proposed ANN model presents a high correlation between the real output and the predicted one. Additionally, the performance of the proposed optimization techniques has been validated by the engineers of the plant, who reported a significant increase in the benefit that was obtained after optimization. Furthermore, this approach has been favorably compared with the results that were provided by a general black-box solver. All methods were tested over real data that were provided by the factory.Ministerio de Ciencia, Innovación y Universidades PGC2018-095322-B-C22Comunidad de Madrid P2018/TCS-4566Unión Europea P2018/TCS-456

The P. aeruginosa effector Tse5 forms membrane pores disrupting the membrane potential of intoxicated bacteria

The type VI secretion system (T6SS) of Pseudomonas aeruginosa injects effector proteins into neighbouring competitors and host cells, providing a fitness advantage that allows this opportunistic nosocomial pathogen to persist and prevail during the onset of infections. However, despite the high clinical relevance of P. aeruginosa, the identity and mode of action of most P. aeruginosa T6SS-dependent effectors remain to be discovered. Here, we report the molecular mechanism of Tse5-CT, the toxic auto-proteolytic product of the P. aeruginosa T6SS exported effector Tse5. Our results demonstrate that Tse5-CT is a pore-forming toxin that can transport ions across the membrane, causing membrane depolarisation and bacterial death. The membrane potential regulates a wide range of essential cellular functions; therefore, membrane depolarisation is an efficient strategy to compete with other microorganisms in polymicrobial environments.We gratefully acknowledge the Laboratories of Dr. Daniel Ladant (Institut Pasteur, Paris) and Dr. Victor de Lorenzo (Centro Nacional de Biotecnologia, Madrid) for the plasmids received (pKTop and pSEVA plasmids, respectively). Also, we would like to acknowledge the Laboratory of Dr. Joseph Mougous for the P. aeruginosa strains received. The technical assistance from Cristina Civantos and Adrian Ruiz is also very much appreciated. We acknowledge the FGCZ for the mass spectrometry analyses and the technical support (Functional Genomics Center Zurich (FGCZ), University/ETH Zurich). D.A.-J. acknowledges support by the MINECO Contracts CTQ2016-76941-R and PID2021-127816NB-I00, Fundacion Biofisica Bizkaia, the Basque Excellence Research Centre (BERC) programme, and IT709-13 and IT1745-22 of the Basque Government, and Fundacion BBVA. A.G.-M. acknowledges the financial support received from the Spanish Ministry of Universities and the Grants for the requalification of the Spanish university system for 2021-2023, financed by the European Union-Next Generation EU-Margarita Salas Modality. A.A. acknowledges support from the Spanish Ministry of Science and Innovation (Project 2019-108434GB-I00 funded by MCIN/AEI/10.13039/501100011033), Generalitat Valenciana (project AICO/2020/066) and Universitat Jaume I (project UJI-B2018-53). M.Q.-M. acknowledges support from the Spanish Ministry of Science and Innovation (Project IJC2018-035283-I funded by MCIN/AEI/10.13039/501100011033) and Universitat Jaume I (project UJI-A2020-21). P.B acknowledges the financial support received from the Spanish Ministry of Science and Innovation through the Ramon y Cajal Programme (contract RYC2019-026551-I)

Desarrollo del prototipo AndaLAND para evaluar suelos agrícolas y agua en el marco del cambio climático

16 páginas.-- 7 figuras.-- 26 referencias.-- Annex 1. Equivalents of USDA and FAO soil classifications (IUSS Working Group WRB 2006)[ES]: There is a critical need to incorporate concerns on soil and water use and protection into land use planning policies and practices, not exclusively in protected areas, especially taking into account the widespread power transfer to local authorities regarding land-use decision making. Accordingly, it is extremely useful what Decision Support Systems (DSS) do on land use. AndaLAND is a prototype DSS which has been developed as a web-based application. It helps to define the vulnerability and the efficiency of the use and protection of soils in the selected target region: Andalusia (Southern Spain). AndaLAND integrates a complete catalogue of information on climate, soil and soil management, to make land vulnerability evaluation. By using Web Map Services (WMS), AndaLAND is fed with available databases from public mapping services (the Geographic Information System for the Common Agricultural Policy-SIGPAC) and other external sources (Microcomputed-based Land Evaluation Information System, MicroLEIS). Climate change scenarios are considered jointly with other relevant global change elements, such as land use change. Soil evaluation is based on decision rules that follow a decision tree. This method was established from Drools (a powerful hybrid reasoning system), using agrological rules for determining soil capacity in plots. The tool is capable of evaluating individually more than 6 million plots currently existing in the Andalusian region. AndaLAND’s final output is a report on the particular vulnerability of a user-specified plot. The report includes information on the plot’s geographical location, its environmental status regarding to (current and potential) impacts, degradation and/or pollution in the water-soil-crop system and eventual affections due to climate change-induced events. The report also provides practical recommendations for sustainable use and management of plots (in particular, for irrigated crops, these recommendations are linked to available irrigation technologies and water consumption).[PO]: Cada vez é mais evidente a necessidade de implementar políticas e práticas que integrem critérios de ordenamento do território para a proteção do uso do solo e da água, não só nas áreas protegidas, tendo em consideração o papel das autoridades locais e dos pequenos agricultores na tomada de decisão sobre os diversos usos do solo. Por isso, e uma vez que existe uma prática cada vez mais difundida no uso de Sistemas de Apoio à Decisão (SAD) para a gestão do uso do solo, o AndaLAND é apresentado como um protótipo dos SAD desenvolvido como uma aplicação web. Esta ferramenta permite determinar a vulnerabilidade e eficiência no uso e proteção do solo na área de estudo: Andaluzia (Sul de Espanha). O AndaLAND integra um catálogo abrangente de informações sobre o clima, solo e gestão da mesma, para permitir a avaliação da vulnerabilidade dos solos alvo. Usando serviços Web Map (WMS), o AndaLAND permite descarregar as informações dos bancos de dados de mapeamento de serviços públicos (Sistema de Informação Geográfica para a Política Agrícola Comum-SIGPAC) assim como de outras fontes diversas (sistemas de informação para a avaliação de serviços web do solo existentes MicroLEIS). Os cenários de alterações climáticas são construídos como informação relevante para prever os potenciais impactos associados com as mudanças no uso do solo. A avaliação global do solo é estabelecida a partir de regras definidas usando árvores de decisão. Este método foi implementado através do sistema Drools (sistema de regras de gestão), utilizando critérios agronómicos para determinar a capacidade agrológica das parcelas. Assim, esta ferramenta é capaz de avaliar individualmente mais de 6 milhões de explorações atualmente existentes na Andaluzia. O resultado final do uso AndaLAND é um relatório individual sobre a vulnerabilidade da parcela definida pelo utilizador como parcela-alvo. Esta documentação inclui informações sobre a localização geográfica da parcela, o seu estado ambiental em relação a atuais e potenciais impactos, possível fase de degradação e / ou contaminação no sistema solo-água-cultura e a robabilidade de ser afetada pelos efeitos associados às alterações climáticas. O relatório final inclui recomendações práticas para a utilização e gestão sustentável na parcela-alvo (especialmente no caso das culturas de regadio estas orientações referem-se às tecnologias de rega existentes e ao consumo de água).[ES]: Cada vez se hace más patente la necesidad de implementar políticas y prácticas de planificación territorial que integren criterios para la protección del uso del suelo y el agua, no sólo en las zonas naturales protegidas, y considerando a su vez el papel desempeñado por las administraciones locales y pequeños agricultores en la toma de decisiones sobre los distintos usos del suelo. Por ello, y dado que existe una práctica cada vez más generalizada en el uso de Sistemas de Ayuda a la Decisión (SAD) para la gestión de los usos del suelo, AndaLAND se presenta como un prototipo de SAD desarrollado como una aplicación web. Esta herramienta permite determinar la vulnerabilidad y la eficiencia en el uso y protección de los suelos en la región objetivo de estudio: Andalucía (S España). AndaLAND integra un catálogo muy completo sobre información climática, de suelos y de manejo de los mismos, para poder realizar la evaluación de la vulnerabilidad de suelos objetivo. Mediante el uso de Web Map Services (WMS), AndaLAND es capaz de volcar la información procedente de las bases de datos de servicios de cartografía públicos (el Sistema de Información Geográfica de la Política Agraria Común-SIGPAC), así como otras fuentes muy diversas (sistemas de información de evaluación de suelos ya existentes en servicios web, MicroLEIS). Los escenarios de cambio climático están incorporados como información relevante para poder predecir los posibles impactos asociados a los cambios de uso del suelo. La evaluación global del suelo se establece a partir de reglas definidas mediante árboles de decisión. Dicho método fue implementado a través del sistema Drools (sistema de gestión de reglas), utilizando criterios agronómicos para determinar la capacidad agrológica en las parcelas. De esta forma, la herramienta es capaz de evaluar individualmente más de 6 millones de explotaciones declaradas actualmente en Andalucía. El resultado final del uso de AndaLAND es un informe individual sobre la vulnerabilidad de la parcela objetivo definida por el usuario. Dicha documentación incluye información sobre la localización geográfica de la parcela, su estado medioambiental con respecto a actuales y potenciales impactos, la posible fase de degradación y/o contaminación en el sistema suelo-agua-cultivo, y la probabilidad de verse afectada por los efectos asociados al cambio climático. El informe final incorpora recomendaciones prácticas para el uso y gestión sostenible en la parcela objetivo (en particular, en el caso de cultivos de regadío estas pautas están referidas a tecnologías de riego existentes y hacia el consumo de agua).This research was funded by the Spanish Ministry of Economy and Competitiveness (MINECO) and the European Regional Development Fund (ERDF) within the framework of the new Precommercial Public Procurement (CPP). The AndaLAND DSS has been jointly developed by the Spanish National Research Council (CSIC), the Institute of Natural Resources and Agrobiology of Seville (IRNAS-CSIC) and two Spanish companies: INCLAM, specialized in water and environmental engineering; and EVENOR-TECH, specialized in Information and Comunication Technologies (ICT) applied to land use planning.Peer reviewe

Prevalence of bovine subclinical mastitis, its etiology and diagnosis of antibiotic resistance of dairy farms in four municipalities of a tropical region of Mexico

A region-wide survey was conducted in the tropical area of Tierra Caliente, State of Guerrero, Mexico to estimate the prevalence of subclinical bovine mastitis (SCM), distribution of mastitis pathogens, and in vitro antimicrobial susceptibility of different mastitis pathogens in dairy farms. In total, 1036 quarter milk samples were obtained from 259 cows at 87 different dairy farms. Collected quarter milk samples were submitted for California Mastitis Test (CMT), bacteriological examination, and testing for antimicrobial susceptibility. Overall prevalence of SCM in the studied area was 20.5 %. Prevalence in the different regions was as follows: 28 % in Arcelia municipality, 21 % in Tlalchapa municipality, 19.4 % in Pungarabato municipality, and 14.3 % in Finch Cutzamala municipality. Of all positive isolates, 97.5 % were Gram-negative bacteria. Moreover, of all positive isolates, 37.5 % were Proteus vulgaris, 25 % Salmonella spp., 12.5 % Enterobacter aerogenes, and 10 % Escherichia coli. Klebsiella pneumonia and E. coli were sensitive for netilmicin antimicrobial. However, E. coli was sensitive for pefloxacin and gentamicin with a sensitivity for pefloxacin for E. aerogenes, while Staphylococci were sensitive for gentamicin and dicloxacillin. It could be concluded that practices such as the implementation of mastitis control programs, improved milking hygiene together with an intramammary treatment with netilmicin, pefloxacin, and gentamicin antimicrobials should be considered for mastitis prevention in the study area of Tierra Caliente, in the tropical area of Guerrero, Mexico

Impact of measurable residual disease by decentralized flow cytometry: a PETHEMA real-world study in 1076 patients with acute myeloid leukemia

The role of decentralized assessment of measurable residual disease (MRD) for risk stratification in acute myeloid leukemia (AML) remains largely unknown, and so it does which methodological aspects are critical to empower the evaluation of MRD with prognostic significance, particularly if using multiparameter flow cytometry (MFC). We analyzed 1076 AML patients in first remission after induction chemotherapy, in whom MRD was evaluated by MFC in local laboratories of 60 Hospitals participating in the PETHEMA registry. We also conducted a survey on technical aspects of MRD testing to determine the impact of methodological heterogeneity in the prognostic value of MFC. Our results confirmed the recommended cutoff of 0.1% to discriminate patients with significantly different cumulative-incidence of relapse (-CIR- HR:0.71, P < 0.001) and overall survival (HR: 0.73, P = 0.001), but uncovered the limited prognostic value of MFC based MRD in multivariate and recursive partitioning models including other clinical, genetic and treatment related factors. Virtually all aspects related with methodological, interpretation, and reporting of MFC based MRD testing impacted in its ability to discriminate patients with different CIR. Thus, this study demonstrated that “real-world” assessment of MRD using MFC is prognostic in patients at first remission, and urges greater standardization for improved risk-stratification toward clinical decisions in AML.This study was supported by the Centro de Investigación Biomédica en Red – Área de Oncología - del Instituto de Salud Carlos III (CIBERONC; CB16/12/00369, CB16/12/00233, CB16/12/00284 and CB16/12/00400), Instituto de Salud Carlos III/Subdirección General de Investigación Sanitaria (FIS No. PI16/01661, PI16/00517 and PI18/01946), Gerencia Regional de Salud de CyL (GRS 1346/A/16) and the Plan de Investigación de la Universidad de Navarra (PIUNA 2014-18). This study was supported internationally by the Cancer Research UK, FCAECC and AIRC under the Accelerator Award Program EDITOR

Current international projects in the Duero and Miño-Sil basins

[EN] This paper summarizes the research work that is being carried out within the framework of three international projects with a lifetime between 2022 and 2026: (1) LIFE-IP-Duero; (2) Supporting stakeholders for adaptive, resilience and sustainable water management; (3) IGCP- 730. (1) and (2) are developed in the Duero river basin and are funded by the European Commission meanwhile (3) is being developed in the Duero and Miño-Sil basins and is funded by the International Geosciences Programme (IGCP) of UNESCO.Peer reviewe

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries